iv) pe anti-mouse igm (rat igg2a; miltenyi 130-095-908  (Miltenyi Biotec)

Journal: BMC Veterinary Research

Article Title: Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida

doi: 10.1186/s12917-025-05233-6

Figure Lengend Snippet: Antibody titers against M. haemolytica and IgG subclass analysis ( n = 10). ( A ) The immunization procedure for titer evaluation. ( B ) BALB/c mice were immunized with rND-white oil, rND-propolis, and rND-Astragalus Polysaccharide Adjuvant, and commercial vaccines, and the serum IgG titers against M. haemolytica were determined. PBS group were used as a control. IgG titer measurements began seven days after the first injection. One-way ANOVA with Dunnett’s multiple comparison test was used to compare the groups. ( C ) IgG2a /IgG1 at OD 450 nm. ( D ) The serum IgG subtype titers against M. haemolytica after the third immunization (IgG1, IgG2a, IgG2b, and IgG3). Two-way ANOVA with Dunnett’s multiple comparison test was used to compare the groups. **** P < 0.0001, * * P < 0.001, ** P < 0.01, * P < 0.05, ns > 0.05

Article Snippet: ProteinIso Ni-NTA Resin was purchased from Beijing All-Golden Biota; Ampicillin(Amp+, 100 mg/ml solution), IPTG(Isopropyl β-D- Thiogalactopyranoside), ELISA coating solution, 5% BSA blocking solution, ELISA termination solution, two-component TMB chromogenic solution, 10× PBST, and 20× TBST were purchased from Beijing Soleibao Technology Co., Ltd.; hRP AffiniPure Goat Anti-Mouse IgG (H + L) from EarthOx; Goat Anti-Mouse IgG1, Human ads-HRP, Goat Anti-Mouse IgG2a, Human ads-HRP, Goat Anti-Mouse IgG2b-HRP and Goat Anti-Mouse IgG3-HRP were purchased from Southern Biotech; the LB medium was purchased from hopebiol Co., Ltd.

Techniques: Adjuvant, Vaccines, Control, Injection, Comparison

Journal: BMC Veterinary Research

Article Title: Antigenicity analysis of the recombinant fusion proteins NlpI of Mannheimia haemolytica and DsbA of Pasteurella multocida

doi: 10.1186/s12917-025-05233-6

Figure Lengend Snippet: Antibody titers against P. multocida and IgG subclass analysis ( n = 10). ( A ) BALB/c mice were immunized with rND-white oil, rND-propolis and rND-astragalus polysaccharide adjuvants, and the serum IgG titers against P.multocida in were determined. PBS group were used as a control. IgG titer measurement began seven days after the first injection. One-way ANOVA with Dunnett’s multiple comparison test was used to compare the groups. ( B ) IgG2a/IgG1 at OD 450 nm. ( C ) The serum IgG subtype titers against P. multocida after the third immunization (IgG1, IgG2a, IgG2b, and IgG3). Two-way ANOVA with Dunnett ‘s multiple comparison test was used to compare the groups. **** P < 0.0001; *** P < 0.001; ** P < 0.01; * P < 0.05; ns, P > 0.05

Article Snippet: ProteinIso Ni-NTA Resin was purchased from Beijing All-Golden Biota; Ampicillin(Amp+, 100 mg/ml solution), IPTG(Isopropyl β-D- Thiogalactopyranoside), ELISA coating solution, 5% BSA blocking solution, ELISA termination solution, two-component TMB chromogenic solution, 10× PBST, and 20× TBST were purchased from Beijing Soleibao Technology Co., Ltd.; hRP AffiniPure Goat Anti-Mouse IgG (H + L) from EarthOx; Goat Anti-Mouse IgG1, Human ads-HRP, Goat Anti-Mouse IgG2a, Human ads-HRP, Goat Anti-Mouse IgG2b-HRP and Goat Anti-Mouse IgG3-HRP were purchased from Southern Biotech; the LB medium was purchased from hopebiol Co., Ltd.

Techniques: Control, Injection, Comparison